Candidate sRNAs validated by Northern blot.

anstSGR group as defined in Table 1: A and B, unique in PAO1 or PA14, respectively; C and E, conserved in both strains but expressed in either PAO1 or PA14, respectively; D, conserved and expressed in both strains.bName or number (e.g. 2326 is PA2326, 30840 is PA14_30840) of loci in the PAO1 and PA14 genomes either overlapping (class II, 5′-UTR; III, asRNA; and V, sense sRNAs overlapping annotated ORFs) or flanking (class I, sRNA) the nstSGRs.cAnnotated sRNAs, found by sRNA-Seq, used as a positive control in Northern blot validation experiments.dUpper (+) or lower (−) genomic DNA strand coincident with cDNA reads.esRNA size predicted by sRNA-Seq. Single values indicates coherent results between PAO1 and PA14. Otherwise, two values (PAO1-PA14) are reported. Values are indicated in bold whenever confirmed by Northern blotting. MRE: Multiple Read Ends defined by non-overlapping reads scattered within the nstSGR. The name of sRNAs used as validation controls is also indicated.fClass assignment in PAO1. The corresponding nstSGR in PA14 was assigned to class V.