Effect of PUS1 knockdown on gene expression in MDA-MB-231 breast cancer cells

To identify the downstream target genes of PUS1 on breast cancer cells, we established MDA-MB-231 cell lines in which PUS1 has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of PUS1 knockdownMDA-MB-231 cells as well as negative control shRNA group Overall design: Compare negative control shRNA and PUS1 knockdown MDA-MB-231 cancer cells using RNA-seq.