Cucumis sativus cultivar:Kfir infected with Pythium spinosum and CGMMV Raw sequence reads

Six days after sowing, 300 seedlings were transplanted into P. spinosum-inoculated medium, and an additional 100 seedlings were transplanted into non-inoculated control medium. Each cucumber seedling was transplanted to a 100 ml pot containing 100 g vermiculite and grown in greenhouse conditions described above. Five days after the P. spinosum inoculation, which permitted observation of the primary "damping off" of seedlings due to Pythium alone (Philosoph et al., 2018), 100 symptomless plants were taken to the core experiment, along with the 100 non-inoculated plants. Fifty plants from each group were then inoculated with CGMMV as described above, forming four different treatments: (i) plants infected with P. spinosum inoculated with CGMMV (PS+CG), plants with P. spinosum (PS); (iii) plants inoculated solely with CGMMV (CG), and (iv) non-inoculated healthy control plants (C).The first collection of samples began on the same day as the CGMMV inoculation (T0); samples were taken from the collar-region (where the hypocotyl meets the root) of 5 cucumber plants inoculated with P. spinosum and five non-inoculated control plants to characterize the plant response before the CGMMV inoculation. Subsequently, the samples were taken from the collar-region of all the four treatments described above (5 plants per treatment) 1, 2, 3, 6 and 13 days post-viral inoculation (dpvi) with CGMMV. All samples were collected in the controlled-environment chamber directly into a test tube with liquid nitrogen and stored at -80 Celsius for later RNA extraction. In addition, the roots of all plants were tested for the presence of P. spinosum using selective corn meal agar.