Knockdown of CDK2AP1 in WA09-hESCs results in EBs with enhanced expression of genes corresponding to the three germ layers.

Quantitative PCR data showing the effect of CDK2AP1 knockdown on the expression of the germ layers markers in EBs derived from WA09 hESCs. EBs obtained from sc-shRNA transduced WA09s and EBs obtained from CDK2AP1-shRNA were harvested and the expression of markers of germ layers (Endoderm: AFP and GATA4. Mesoderm: T and IGF2. Ectoderm: SOX1 and NESTIN) was examined by qPCR. Knockdown of CDK2AP1 significantly enhanced the expression of all markers of differentiation (p-value < 0.05).