File S1 - Molecular Variation in <i>AVP</i> and <i>AVPR1a</i> in New World Monkeys (Primates, Platyrrhini): Evolution and Implications for Social Monogamy

Contains Table S1, Sample information for the New World monkeys in this study. Table S2, PCR primers used to amplify genomic coding regions of AVP and AVPR1a (Underlined primer are the nested primers). Table S3, Radical or conservative change for each substitution in AVPR1a of NWM. N-term, N-terminus; TM, transmembrane region; IC, intracellular region; C-term, C-terminus. Reference sequence is human AVPR1a. Figure S1, Alignment of AVPR1a amino acid substitutions in NWM (shaded) and non-NWM relative to human AVPR1a. Green indicates NWM-specific substitutions, red represents unique substitutions in marmoset and tamarin, yellow indicates the substitution in marmoset and titi monkeys, blue indicates the marmoset-specific substitution, and ‘.’ represents identity with human. The vertical numbers in this figure indicate the amino acid position in AVRP 1a protein. AVPR1a sequences of Tarsius syrichta and Otolemur garnettii were not available from public data. The numbers of potential positive selection sites are framed. * indicates social monogamy. Figure S2, Alignment of the AVPR1a predicted amino acids in primates. The dot implicates the identity with human; pink indicates the extracellular regions; yellow represents the transmembrane regions; and blue indicates the intracellular regions. NWM are framed in the figure. Figure S3, Sliding window analysis of the dN/dS ratio and dN value along AVPR1a gene. A. Microcebus and 17 NWM genera. B. Microcebus and Hominoid. C. Old World monkeys against Microcebus. The ratio/value are drawn over the midpoint window position (window length 50, step size 10) from whole coding region. The elements of AVPR1a are highlighted in red (extracellular), yellow (transmembrane), and green (intracellular). (DOCX)