Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for
*χ2-test, P<0.01.Percentage of live pups (%) = No. live pups/No. embryos* 100%; Percentage of herniated pups(%) = No. herniated pups/No. live pups*100%; Co-injection of 2nESCs and 4nESCs into tetraplo
