Development of gene expression signatures for WT (Hif1afl/fl) mice and VSMC-specific HIF1α disruption (Hif1a△SMC) mice VSMCs treated with 150μM CoCl2 for 24hours

HIF1α is a key regulator of hypoxia, however, the changes of its target genes in VSMCs under hypoxia were unknown. CoCl2 is a chemical hypoxia agent which leads to the stabilization of HIF1-α and the expression of hypoxia responsive genes, Therefore Hif1afl/fl and Hif1a△SMC VSMCs were treated with CoCl2 and performed microarray analysis. We used microarray to detail the gene expression of WT (Hif1afl/fl) mice and VSMC-specific HIF1α disruption (Hif1a△SMC) mice VSMCs treated with 150μM CoCl2 for 24hours. RNA extraction from WT (Hif1afl/fl) mice and VSMC-specific HIF1α disruption (Hif1a△SMC) mice VSMCs treated with 150μM CoCl2 or an equal volume of PBS for 24 hours and hybridization on Agilent microarrays. The VSMCs were devided into four groups: 1. Hif1afl/fl VSMCs treated with PBS for 24 hours, 2. Hif1a△SMC VSMCs treated with PBS for 24 hours, 3. Hif1afl/fl VSMCs treated with CoCl2 for 24 hours, 4.Hif1a△SMC VSMCs treated with CoCl2 for 24 hours.