Inhibition of KT71^T cultures growing on different substrates by the addition of fluoroacetate.

Fluoroacetate was added to cultures at the beginning of the exponentially growth phase. All cultures were incubated at 28°C with an initial oxygen concentration of 12 vol% under dim light. The growth inhibition caused by a distinct amount of fluoroacetate compared to growth in normal medium was calculated by using the following formula: Inhibition (%) = [1−(A660 ts−A660 t0)FAc/(A660 ts−A660 t0)]×100, where A660 is absorption at 660 nm, ts the time at which the culture without fluoroacetate reached the stationary phase, t0 the time at which fluoroacetate was added and FAc designates the culture supplemented with fluoroacetate. n.d., not determined.