miR-146a Suppresses SUMO1 Expression and Induces Cardiac Dysfunction in Maladaptive Hypertrophy

Table I. Calcium transient and
contractility parameters of cardiomyocytes infected with Ad_pre-mir-146a or
Ad_decoy-146a.

Top table,
isolated mouse cardiomyocytes were infected with Ad_pre-mir-146a (MOI=50) for
24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts).

Bottom table,
isolated mouse cardiomyocytes were infected with Ad_decoy-146a (MOI=50) for
24h. Data represent the mean ± s.e.m. in all panels (n=100-150 cells/4 hearts).
Table II. Echocardiographic parameters of
mice injected with rAAV9_pre-mir-146a.

Data represent the mean ± s.e.m.
of cardiac functional parameters 4 weeks post-injection.
Table III. Hemodynamic parameters of mice
injected with rAAV9_pre-mir-146a.

Data represent the mean ± s.e.m.
of cardiac functional parameters 4 weeks post-injection. Max dPdt, maximum
dP/dt; Min dPdt, minimum dP/dt.
Table IV. Echocardiographic parameters of
mice injected with rAAV9_decoy-146a.

Data represent the mean ± s.e.m.
of cardiac functional parameters 4 weeks post-injection.
Table V. Hemodynamic parameters of mice
injected with rAAV9_decoy-146a.

Data represent the mean ± s.e.m.
of cardiac functional parameters 4 weeks post-injection.
Table VI. Echocardiographic parameters of
SUMO1 TG mice injected with rAAV9_pre-mir-146a.

Data represent the mean ± s.e.m. of cardiac functional
parameters 8 weeks post-injection. NL, Negative littermate; SUMO1 TG, Cardiac-specific
Cre/loxP-conditional Sumo1-transgenic
mice
Table VII. Cardiac cellular composition.

Isolated cardiac cells were
sorted with FACS and analyzed. Data represent the mean ± s.e.m. (n=5).