Ultra-deep bulk RNA-seq from hESC-derived thyroid follicular cells

This study provides ultra-deep bulk RNA-seq data from human embryonic stem cell (hESC)-derived thyroid follicular cells. The NKX2-1GFP+ population was isolated using fluorescence-activated cell sorting (FACS) and subjected to RNA extraction. Library preparation was performed using the Ovation Solo RNA-seq System, followed by high-output sequencing on the Illumina HiSeq 1500 platform. Each sample generated approximately 400 million reads. These data enable transcriptomic analysis of differentiated thyroid lineage cells exposed to IFN-a and IFN-?. Overall design: Human embryonic stem cell-derived thyroid follicular cells were differentiated in vitro. NKX2-1GFP+ cells were isolated using FACS and immediately lysed in Qiazol. Total RNA was extracted using the miRNeasy Micro Kit. Libraries were prepared with the Ovation Solo RNA-seq System (NuGen), and sequencing was performed on the Illumina HiSeq 1500 platform, generating ~400 million reads per sample. The experimental conditions included IFN-a, IFN-?, and untreated controls. The dataset supports transcriptome profiling and differential expression analysis between these groups.