Shuffling the yeast genome using CRISPR/Cas9-generated DSBs that target the transposable Ty1 elements

We explored how Cas9-induced double-strand breaks (DSBs) on Ty1 produce genomic alterations in the diploid yeast Saccharomyces cerevisiae. Following Cas9 induction, we observed a significant elevation of chromosome rearrangements (large deletions and duplications), loss of heterozygosity (gene conversions, crossovers, and break-induced replication), and aneuploidy. Almost all of the chromosomal rearrangements reflect the repairing of DSBs at Ty1 elements by homologous recombination.本研究探讨了二倍体酿酒酵母（Saccharomyces cerevisiae）中，Cas9诱导的Ty1位点双链断裂（double-strand breaks, DSBs）如何引发基因组变异。在Cas9诱导处理后，我们观测到染色体重排（大片段缺失与重复）、杂合性缺失（基因转换、交叉互换及断裂诱导复制）以及非整倍体的水平显著升高。几乎所有的染色体重排均反映了通过同源重组修复Ty1元件处双链断裂的过程。