RNA-seq of regenerating hydra vulgaris tissue with ERK inhibition

Elucidating the gene regulatory network downstream of ERK signaling after injury. This dataset contains 3 biological replicates of uninjured whole animals, 0, 1.5, 3, 8, 12 hours post amputation Hydra regenerating tips that were treated with 25uM U0126 or DMSO (vehicle control). The data were collected in two separate experiments. The files labelled with A, B, C, and W are from 2024, while the H, F files are from 2023. Batch correction is necessary.------The SRA metadeta contains a unified naming scheme of [regenerating tissue][drug][timepoint][biorep]. 0hpa bioreps are in both datasets, so they are separated with _1 and _2. Use SRA metadata to rename all files if you are working with both datasets.------2023 dataset (H, F)File naming scheme: [regenerating tissue][drug][timepoint][biorep]H= regenerating headF= regenerating footD= DMSO treatedU= U0126 treatedThe number following D or U is the regeneration timepoint. 0 is 0 hours post amputation (hpa), 5 is 1.5 hpa, 3 is 3hpa.------2024 dataset (A, B, C)File naming scheme: [timepoint][drug][biorep]All data is from regenerating head.A is 0hpa, B is 8hpa, C is 12hpa. W = whole animals treated for 12 hours.D= DMSO treatedU= U0126 treated