pool-seq of Drosophila melanogaster in starvation cage experiment

pool-seq of Drosophila melanogaster in starvation cage experiment. 200 iso-female strains were used to represent the natural populations of D. melanogaster in China. Ten male adults at the age of 3-5 days after eclosion were collected from each strain and pooled into a population cage with cornmeal food to acclimate for one day. The medium was replaced by 1.5% agar to start the starvation stress. Dead individuals were collected from the cage and quickly frozen in liquid nitrogen with a time interval of 6 or 12 h. The experiments were performed for two replicates. Individuals were grouped based on the survival time into seven samples. Seven representative samples with about 200 individuals from each replicate were sequenced to a depth of about 200X.