The dominant negative effect of CIII mutants as observed by plaque morphology.
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AD16 strains carrying plasmids expressing wild type and mutated CIII via tac promoter were grown overnight at 32°C in LB or LB supplemented with 100 µg/ml ampicillin. A lawn of each strain was plated on TB plates in the presence of 10 µM IPTG and then spotted by different concentrations of the phages indicated in the table. C-clear plaques, T-turbid plaques.
创建时间：
2013-02-21
