RNA-Seq of kidney glomeruli and renal tubules from Type 2 diabetic (db/db) and non-diabetic mice

We investigated the gene expression profiles of RNA isolated from kidney glomeruli and renal tubules from aged, 24 week old type-2 diabetic (db/db) and non-diabetic mice In order to investigate the consequences of hyperglycemia on the pathogenesis and progression of diabetic nephropathy Kidney glomeruli and renal tubules, glomeruli and renal tubules were isolated from 9 db/db mice and 9 db/m mice. Each sample included glomeruli from 3 mice, and three independent biological replicate samples were sequenced from each group. RNA was purified for RNA-Seq analysis on the Illumina HiSeq 4000. The goal of the project was to generate comprehensive list of RNA genes differentially regulated between the two conditions in order to identify novel targets for further study.