Genetic Characteristics and Phylogenetic Relationships of 18 Anchovy Species Based on Mitochondrial Genomes in the Seas Around China

We downloaded the complete mitochondrial genome data of 18 Engraulidae fish species from the NCBI database (https://www.ncbi.nlm.nih.gov/). These files were stored in the “Download data” folder. Subsequently, we reannotated these mitochondrial genomes using the MITOS2 online tool available on the Galaxy website (https://usegalaxy.org/) and manually modified the original gb files to adjust the inaccurately annotated control regions and to add the annotation information for the light-strand replication origin. The revised files were saved in the “Reannotation” folder and were used for subsequent analyses.