In vitro signaling properties of cannabinoid and orexin receptors: how orexin receptors influence cannabinoid receptor-mediated signaling

The co-expression of different types of G protein-coupled receptors (GPCRs) in the same cells can have implications on receptor signaling and receptor cross-talk, potentially altering the apparent potency or efficacy of ligands targeting each receptor. The endocannabinoid and orexinergic systems, consisting of class A GPCRs and their endogenous ligands, are highly complex and regulate processes such as appetite, sleep, nociception, and energy homeostasis. The shared anatomical distribution of cannabinoid and orexin receptors in various regions of the central nervous system (CNS), coupled with data from previous studies exploring physical and functional interactions between these receptors, suggests that the endocannabinoid and orexinergic systems engage in crosstalk. In this study, we explored how orexin receptors (OX1, OX2) altered the in vitro signaling of cannabinoid receptors (CB1, CB2) in Chinese hamster ovary (CHO)-K1 cells by quantifying cyclic adenosine monophosphate (cAMP) inhi..., , , # Data from: In vitro signaling properties of cannabinoid and orexin receptors: how orexin receptors influence cannabinoid receptor-mediated signaling

[https://doi.org/10.5061/dryad.0cfxpnwcq](https://doi.org/10.5061/dryad.0cfxpnwcq)

## Description of the data and file structure

File name: 2024-10-03_RawData_Manuscript_PharmacolResPersp_update.xlsx

This Dryad submission includes an Excel file containing raw data and analyses related to the linked manuscript. Data are from:

(1) Fluorescent microscopy assays that have been analyzed for the number of nuclei (Hoescht staining) and the number of green fluorescent protein (GFP)-positive cells where either the orexin 1 or orexin 2 receptor is tagged with GFP. 

(2) cAMP inhibition assays that have been analyzed for cAMP inhibition following treatment of Chinese hamster ovary (CHO)- K1 HitHunter (DiscoveRx) cells stably expressing either the type 1 or type 2 cannabinoid receptor (CB1R, CB2R) and transfected with either orexin 1 or 2 (OX1, ...,