Additional file 1: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Table S1. Sequences of reagents used in the study. The table shows the sequences of the oligonucleotides and lssDNA donors, primers and TaqMan assays employed in this study. LoxP sites (for all conditional projects) and point mutations (for Gckr and Rims1 project) are underlined. Sequences added for diagnostic (for all conditional projects except Syt7) and silent mutations (for Gckr and Rims1 project) are shown in italics. For the plasmids, sequences flanked by and including homology arms are shown. The ddPCR reference copy counting assay is labelled with VIC. All other ddPCR copy counting assays are labelled with fluorescein amidite (FAM). Copy counting assays labelled as UNIV ddPCR assays recognize both WT and engineered alleles; MUT ddPCR assays recognize engineered allele only. Table S2. Production of founders for conditional alleles. The table shows the numbers of embryos and animals involved in mutagenesis attempts employing the injection of CRISPR/Cas9 reagents and lssDNA donors. Table S3. Generation of conditional alleles employing different donor types. The table shows the numbers of embryos and animals involved in mutagenesis attempts employing the injection of CRISPR/Cas9 reagents and oligonucleotides, plasmids or lssDNA donors. The results of the analysis of the founders obtained from these attempts are also summarized. Table S4. Generation of a Rims1R655H point mutation. Further genotype screening data for this project are shown in Additional file 18: Figure S16 and Additional file 19: Figure S17. Table S5. Analysis of the Rims1R655H project. The table details the results of screening of five positive F0 animals obtained for the generation of a Rims1R655H point mutation and the subsequent characterization of the F1 animals obtained from mating of these F0 animals to WT mice. Table S6. Nomenclature of new mouse lines established in the course of the study. (XLS 81 kb)