IFNγ induces degradation of LDs in Irgm1/m3−/− MEFs.

Cells were cultured overnight in the absence or presence of IFNγ and in the absence of OA. 12 hours post IFNγ treatment, BAF was added to the culture media as indicated for an additional 8–12 hours and the BODIPY signal was measured by flow cyometry. Flow cytometry histograms of BODIPY-labeled wildtype and Irgm1/m3−/− MEFs representative of three independent experiments are shown. The fold change in the average mean fluorescent intensity (MFI) in response to IFNγ treatment is plotted in the panel on the right (***, p