Genotype of transconjugants that escape CRISPR immunity.

(a)Transconjugants obtained in two independent experiments (R and B) are shown. Transconjugants R21 and R26 are not reported as genotypic analysis indicated these were S. aureus RN4220/pG0400 donors that acquired neomycin resistance. Highlighted and underlined are transconjugants that were chosen for the pairwise competition experiments estimating fitness.
(b)The number indicates the adenine residue deleted relative to the start codon of the gene.
(c)The number indicates the position of insertion of the IS256 element relative to the start codon of the gene. Direct, d, or inverted, i, refer to the orientation of the transposase gene of the IS256 element with respect to the direction of transcription of the cas/csm operon.
(d)The numbers indicate the chromosomal coordinates of the S. epidermidis RP62a genome that are deleted. The elements that are believed to have mediated the deletion are shown in parenthesis.
(e)Deletion of the adenine in the first position of the spc1 sequence.
(f)The nucleotide mutation followed by the amino acid mutation are indicated, the numbers indicated nucleotide or amino acid position of the gene or encoded protein, relative to the start codon or initial methionine residue, respectively.
(g)Highlighted and underlined are transconjugants that were chosen for the pairwise competition experiments estimating fitness.