Transcriptome Analysis of Diffuse Large B Cell Lymphoma Cells Inducibly Expressing MyD88 L265P Mutation Identifies Upregulated CD44, LGALS3, NFKBIZ, and BATF as Downstream Targets of Oncogenic NF-?B Signaling

In this study, we used a doxycycline (DOX) inducible gene expression system to introduce MyD88, a gene associated with lymphoma, into the U2932 lymphoma cell line. We performed a transcriptomic analysis (RNA-seq) to identify differentially expressed genes due to the MyD88 L265P oncogenic mutation. This study aimed to investigate the impact of MyD88 L265P oncogenic signaling on lymphoma cells by analyzing the transcriptomic response of model cell lines. Overall design: To introduce MyD88 wt or L265P into the cells, we transduced the U2932 cell line with lentiviral particles containing the pLVX_TetOne inducible system. After 24 hours of induction with DOX hyclate, we harvested the cells and extracted the total RNA. At least 2 µg of total RNA was sent to Macrogen Europe for TruSeq stranded mRNA library generation and RNA-seq analysis on a NovaSeq 6000 Illumina platform. We used a total of 12 samples, with 3 samples for each condition (MyD88 wt -/+ DOX and MyD88 L265P -/+ DOX).