Global database of DNA barcoding marker genes available for third-party curation

This dataset comprises eukaryotic DNA barcoding marker genes (rbcL, COI for Fungi, matK, TEF-1α) available for third-party curation, download, and analysis in PlutoF (https://plutof.ut.ee). These marker gene sequences were obtained from the International Nucleotide Sequence Database Collaboration (INSDC; https://www.insdc.org/) using custom search filters in NCBI E-utilities programs and will be continuously updated. List of marker genes includes:

1. rbcL (Ribulose-1,5-bisphosphate carboxylase/oxygenase)
2. COI (mitochondrial cytochrome C oxidase)
3. matK (maturase K gene)
4. TEF-1α (translation elongation factor 1-alpha)

Details on search filters and downloaded sequence counts are provided in the attached CSV file. Additionally, the dataset contains sequences from 18S and 28S ribosomal RNA for all eukaryotes, acquired during a pilot phase of this project. Nucleotide sequences and their source metadata, which are part of this dataset, can be curated using the 'third-party curation of DNA sequences' services in PlutoF developed as part of the BiCIKL project (https://bicikl-project.eu/).

The database can be accessed in PlutoF at https://app.plutof.ut.ee/study/view/108969.