mTORC1 activity suppresses ferroptosis through a SCARB1-dependent HDL-tocopherol uptake pathway

Study of the relationship between mTORC1 and ferroptosis, revealing that mTORC1 regulates SCARB1 expression and thus governs SCARB1 interaction with HDL and the uptake and delivery of HDL-derived tocopherol.

Immunoblotting folder:
Uncropped immunoblots from Figures 1A, 2G, 4B, 4E, 5B, S2C, S2G, and S4B.

Immunoblots indicate alterations in mTOR signalling, successful CRISPRi or CRISPRa perturbation of target genes, and alterations in SCARB1 expression upon mTOR inhibition, as outlined in greater detail in the linked manuscript.

Microscopy folder:
Imaging of HDL uptake ± mTORC1 inhibitor RM-006 from Figure 5H - confocal images demonstrate defective uptake of Dil-HDL into lysosomes in mTORC1-inhibited cells.

Display and raw images are provided in the relevant subfolders - raw image file labels are ‘condition_replicate_LAMP488_DilHDL568_image#’ and include a blue Hoechst DNA channel, a green LAMP1 AF488 channel, and a red Dil-HDL AF568 channel.