Enhanced metabolic detoxification is associated with fluroxypyr resistance in Bassia scoparia

Resistance to the synthetic auxin herbicide fluroxypyr has an unconfirmed non-target site resistance mechanism. An RNA-Seq experiment was used to align to a reference transcriptome for the weed species Bassia scoparia to quantify gene expression and to identify sequence variants in the alignments. High gene expression was found in several genes related to metabolic degredation of the parent fluroxypyr compound. Young leaves were sampled for RNA extraction from four individuals from a resistant line (B3) and two susceptible lines (9425 and J01). RNA was used for preparation of Illumina Hi-Seq libraries. Samples were collected from untreated plants sprayed with water, and the same plants treated with fluroxypyr 3 and 10 hours after treatment