Accumulation of BaMV, satBaMV, and siRNAs in leaves of <i>N. benthamiana</i> and <i>A. thaliana</i>.

Total RNA of 2.5 µg from N. benthamiana or 5 µg from A. thaliana were analyzed by northern blot analysis. The BaMV genomic (6.4 Kb), two subgenomic RNAs (2.0 and 1.0 Kb) and vsiRNAs were detected by a BaMV-specific probe and satRNA and satsiRNAs by a satBaMV-specific probe. M: mock (water) inoculation; −: BaMV alone. F4: noninterfering BSF4 satBaMV; L6: interfering BSL6 satBaMV. All films were exposed overnight except for detection of BaMV in N. benthamiana systemic leaves infected with BaMV or co-infected with BSF4 and BSL6 (3-hr exposure).