Genome-wide maps of FOXO1 binding sites in human endothelial cells

ChIP-Seq profiling of human umbilical vein endothelial cells (HUVECs) overexpressing constitutively active FOXO1 to identify FOXO1 DNA binding sites and to assess changes in the histone modifications H3K4me3 and H3K27ac. HUVECs with or without constitutively active FOXO1 were analyzed 24 h post adenoviral transduction. FOXO1 binding and the histone modifications H3K4me3 and H3K27ac were analyzed by ChIP-seq with antibodies targeting FOXO1, H3K4me3 and H3K27ac.