Structure of the carbohydrate compounds used for primary and secondary screening and number of hit clones isolated from the fecal (F) and the ileum mucosa (I) libraries.
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aXOS, xylo-oligosaccharides; FOS, fructo-oligosaccharides; GOS, galacto-oligosaccharides. bClones positive on fructo-oligosaccharides were further assayed for inulin degrading activity. cClones positive on lactulose (I library) and those identified by screening the entire F library (136, 000 clones) on AZCL-galactan were further assayed on galacto-oligosaccharides. dNT: non tested. eScreening steps: 1, high-throughput primary screen; 2, HPAEC-PAD based secondary screen.
创建时间：
2013-09-16
