Expression data from mice neonatal pancreas (P1, same litter as control and Elastase-Cre mediated Pdx1cKO)

During embryogenesis, exocrine and endocrine pancreatic tissues are formed in distinct regions within the branched ductal structure in mice. We previously reported that exocrine-specific inactivation of Pdx1, an indispensable gene for pancreatogenesis, by Elastase-Cre caused not only hypoplastic exocrine formation but also substantial endocrine defects resulting in diabetic phenotype, indicating the existence of exocrine-driven factor(s) that regulate proper endocrine development. We obtained pancreata from control and mutant mice at P1 and subjected to microarray analysis. To obtain Pdx1-conditional knock-out mice, Pdx1loxP mice and Elastase-Cre transgenic mice were interbred. Pancreata were dissected from postneonatal day 1 and immediately submerged in RNAlater (Ambion, Inc., Austin, TX) and subjected to microarray analysis.