VMP1 restricts NLRP3 inflammasome activation through its modulation of SERCA activity and autophagy

To investigate the effect of VMP1 deletion on gene expression in either untreated or LPS and ATP treated human differentiated THP-1 cells. CRISPR-Cas9 technology was used to generate stable knockout cell lines. We then performed an RNA-Seq experiment to assess gene expression changes between untreated and treated VMP1 KO or control macrophages. Comparative gene expression profiling analysis of RNA-seq data for THP-1 cells and its KO derivatives (VMP1 KO). Three replicates were included for each condition: untreated differentiated control THP-1s, LPS and ATP treated differentiated control THP-1s, untreated differentiated VMP1 KO THP-1s, and LPS and ATP treated differentiated VMP1 KO THP-1s.