Comprehensive transcriptome analysis of the Mettl18-deficient embryos

Histidine methylation is an evolutionarily conserved but poorly understood post-translational modification. METTL18 is known to catalyze histidine Nt-methylation in vitro, yet its physiological functions in mammals remain unclear. In this study, we performed a comprehensive analysis of gene expression changes in embryos of wild-type and Mettl18-deficient mice using RNA sequencing (RNA-seq). Overall design: mRNA profiles of the whole worm were generated by RNA sequencing using the NextSeq 500 (Illumina).