Curated non-redundant repeat library of <i>Gomphocerus sibiricus</i>
收藏资源简介:
We used Earl Grey v5.1.0 (Baril et al. 2024) to predict repetitive DNA sequences <i>de novo</i> in the new chromosome-level assembly and retrieve a non-redundant species-specific repeat library. We used the repeatlib_filtering_workflow to filter the repeat library for non-TEs resembling proteins (source code: https://github.com/NBISweden/repeatlib_filtering_workflow) and parsed the filtered repeat library through TEtrimmer (Qian et al. 2024) to automate the manual curation of the repeat library. The curated library is currently being submitted to the Dfam database (Storer et al. 2021).
本研究采用Earl Grey v5.1.0(Baril等,2024)在新构建的染色体级基因组组装序列中从头预测重复DNA序列,并获取非冗余的物种特异性重复序列文库。我们借助repeatlib_filtering_workflow对该文库进行过滤,以去除其中类似蛋白质的非转座因子(non-TEs),其源代码公开于:https://github.com/NBISweden/repeatlib_filtering_workflow。随后通过TEtrimmer(Qian等,2024)处理过滤后的重复序列文库,实现该文库手动注释流程的自动化。目前,经人工优化注释的重复序列文库已提交至Dfam数据库(Storer等,2021)。




