Detection of off-target sites in the AAVS1 site
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To examine whether there were off-target effects in the 293T cell genome, we identified other potential off-target sgRNA sequences for Cas9 [27]. These off-target sequences all end before the NGG base and have the best match with the guide RNA. Subsequently, we selected the six high-probability off-target sites as shown in Figure S2D. However, no mutated indel sequences were detected upon alignment with NCBI reference sequences, indicating no off-target effects in this cell line.
创建时间：
2024-10-23
