Fusion of different epitope tags to N-terminus of BST-2 ΔN20 results in different effects on intracellular trafficking.
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https://figshare.com/articles/dataset/_Fusion_of_different_epitope_tags_to_N_terminus_of_BST_2_916_N20_results_in_different_effects_on_intracellular_trafficking_/1219007
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(A) 293T cells in 10-cm plates were respectively transfected with BST-2 ΔN20 NT, BST-2 ΔN20 NHA, BST-2 ΔN20 NMyc, BST-2 ΔN20 NFlag, BST-2 ΔN20 NHARD and the cell lysates were analyzed by sucrose gradient ultracentrifugation. Samples were analyzed by Western blotting with an anti-BST-2 pAb. The major bands corresponding to higher and lower glycosylated BST-2 are marked with symbols. The level of glycosylated forms of BST-2 in each sample was respectively quantified and presented in line arts. (B) The cell surface expression of BST-2 ΔN20 NMyc, BST-2 ΔN20 NFlag and BST-2 ΔN20 NHARD were analyzed by the FACS assay as described above in Figure. 5. This experiment was repeated for three times and the most representative data was shown.
创建时间：
2016-02-23
