Transcriptional profiling sheds light on the cellular and fibrotic aspects of idiopathic subglottic tracheal stenosis. Transcriptional profiling sheds light on the cellular and fibrotic aspects of idiopathic subglottic tracheal stenosis

Transcriptional analysis of idiopathic subglottic tracheal stenosis Overall design: Single cell RNA-sequencing gene expression comparison of subglottic tracheal stenosis with healthy trachea. Single-cell suspension from all samples were prepared using MACS Milteny Multi Tissue Dissociation Kit 1 (Miltenyi). Libraries were generated using Chromium Next GEM Single Cell 5´GEM, Library & Gel Bead Kit v5.2, Chromium Next GEM Chip K Single Cell Kit and Single Index Kit TT Set A (all 10x Genomics, Pleasanton, CA, USA).