Transcriptomic changes in the mouse foregut comparing Gli2,3 double mutant to heterozygous controls

We dissected foreguts from Gli2-/-,Gli3-/- and Gli2+/-,Gli3+/- mouse embryos at embryonic day 9.5. Three foreguts were obtained for each group and each foregut was individually processed for bulk RNA extraction and library preparation. Sequencing results identified Gli positively and negatively regulated genes in the foreguts. Examination of transcriptomic changes in the mouse foregut comparing Gli2,3 double mutant to heterozygous controls