Proliferation of hAEC that display pluripotent markers <i>in vitro</i>.

(A) Representative micrographs at 20X from hAEC at different passages (P0-P3) immunostained for OCT4 (red), SOX2 (red), NANOG (red), SSEA3 (green), SSEA4 (green), TRA-1-60 (green) and E-cadherin (green) and also for Ki67 (green or red); the nuclei were stained with DAPI (blue). (B) Graph shows the percentage of hAEC positive for one of the pluripotency factors and Ki67. Results are expressed as percentages of means ± S.E.M. from 9 fields counted in duplicate from five independent experiments. Scale bar 50 μm.