Data_Sheet_1_Comparative Proteomic Analysis of Different Parts of Taenia Hydatigena.xls

Taenia hydatigena, a globally distributed parasite, is a canine tapeworm and causes huge economic losses in the food industry. Using LC-MS/MS, the proteomes of T. hydatigena cyst scolex, designated as CS, and the cyst without the scolex, designated as CWS, were profiled and a total of 764 different proteins were identified, 664 of which were identified in CS, 412 identified in CWS, and 312 in both. Comparative analysis revealed that CS had more abundant proteins associated with growth and development, while CWS had more abundant proteins constituting a scaffolding and protective extracellular matrix. Consistent with the sequencing data, the abundance of the five selected proteins was validated to be higher in CWS than CS by Western blotting. The current data will provide a clue for further pinpointing a role of these proteins in the biology of T. hydatigena.

Taenia hydatigena，一种在全球范围内广泛分布的寄生虫，属于犬类绦虫，在食品行业中造成了巨大的经济损失。通过液相色谱-串联质谱（LC-MS/MS）技术，对T. hydatigena囊虫的吸盘（命名为CS）和不含吸盘的囊虫（命名为CWS）的蛋白质组进行了分析，共鉴定出764种不同的蛋白质，其中664种在CS中鉴定到，412种在CWS中鉴定到，312种在两者中均有发现。比较分析表明，CS中与生长和发育相关的蛋白质更为丰富，而CWS中构成支架和保护性细胞外基质的蛋白质更为丰富。与测序数据一致，通过蛋白质印迹法（Western blotting）验证了五种选定的蛋白质在CWS中的丰度高于CS。当前数据将为进一步精确指认这些蛋白质在T. hydatigena生物学中的作用提供线索。