Lumbar spinal cords: Sham rats vs. chronic constriction injury (CCI) induced chronic neuropathic pain rats. Lumbar spinal cords: Sham rats vs. chronic constriction injury (CCI) induced chronic neuropathic pain rats

To investigate the role of DNA methylation in modulating chronic neuropathic pain (NPP), and identify possible target genes of DNA methylation involved in this process. The chronic constriction injury (CCI) induced NPP model was used. The Arraystar Rat RefSeq Promoter Arrays were used to identify the methylation profiles at the genome-wide level in the DNA promoter regions of the lumbar spinal cord in rats 14 Days following CCI surgery. The underlying genes with differential methylation were then identified and submitted to Gene Ontology and pathway analysis. Methyl-DNA immunoprecipitation quantitative PCR (MeDIP-qPCR) and quantitative reverse transcription-PCR (RT‒qPCR) were used to confirm gene methylation and expression. Overall design: Two-condition experiment, lumbar spinal cord in sham rats vs. CCI rats. Biological replicates: 3 sham replicates, 3 CCI replicates.