Clathrin recruits auxilins to the clathrin-coated vesicle

After fission from the plasma membrane, auxilin proteins DNAJC6 and GAK are recruited to the vesicle through interaction with clathrin and phosphoinositides, in particular PI4P (Greener et al, 2000; Lee et al, 2006; Massol et al, 2006; Taylor et al, 2011; Scheele et al, 2001; Fotin et al, 2004a; Fotin et al, 2004b; Guan et al, 2010; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015). Auxilin in turn recruits the ATPase HSPA8 (also known as HSC70) , which uses the energy from ATP hydrolysis to remove the clathrin-coat from the vesicle, priming it for fusion with a subsequent endosomal compartment (Schlossman et al, 1984; Ungewickell et al, 1995; Rappoport et al, 2008; Xing et al, 2010; Bocking et al, 2011; Rothnie et al, 2011; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015).

在质膜裂解之后，辅助蛋白DNAJC6和GAK通过与网格蛋白和磷脂酰肌醇（尤其是PI4P）相互作用而被募集至囊泡中（Greener等，2000；Lee等，2006；Massol等，2006；Taylor等，2011；Scheele等，2001；Fotin等，2004a；Fotin等，2004b；Guan等，2010；参见McMahon和Boucrot，2011；Sousa和Lafer，2015综述）。随后，辅助蛋白进一步募集ATP酶HSPA8（亦称HSC70），该酶利用ATP水解的能量移除囊泡上的网格蛋白衣，为其与后续的细胞内体室融合做准备（Schlossman等，1984；Ungewickell等，1995；Rappoport等，2008；Xing等，2010；Bocking等，2011；Rothnie等，2011；参见McMahon和Boucrot，2011；Sousa和Lafer，2015综述）。