Assessment of the Pol IV largest subunit, NRPD1, DeCL domain deletion construct to rescue Pol IV-dependent DNA methylation in the nrpd1-3 mutant (Bisulfite-Seq).

The Arabidopsis thaliana multi-subunit RNA Polymerase IV largest subunit DeCL domain was functionally assessed with an NRPD1 DeCL domain deletion construct. The Pol IV largest subunit DeCL domain deletion mutant, nrpd1 ΔDeCL, was assessed for Pol IV-dependent DNA methylation with whole genome bisulfite sequencing.