Respiration rates and respiratory control ratios for permeabilized neurons oxidizing various substrates.

Initial, state 3, state 4, and uncoupled O2 consumption rates (OCRs) are expressed in pmol O2/min/80,000 plated cells. The initial OCRs are the rates measured just prior to permeabilization or control injection (intact). The state 4 rates were measured in the presence of oligomycin. The respiratory control ratio (RCR) is the ratio of the state 3 rate to the state 4 rate, calculated on an individual well basis prior to averaging. Data are mean ± SE, n = 4 experiments of 3–5 wells per treatment. One well (P/M) was excluded from analysis because the antimycin OCR was higher than the oligomycin OCR.*indicates a significant difference compared to intact cells (p