Induction of a colitogenic phenotype in Th1-like cells depends on interleukin-23 receptor signaling [cd160 knockout ex-vivo]

RNA-sequencing of wild-type and cd160 knockout CD4 T cells utilized in a T cell transfer colitis model Overall design: Naïve CD4+ CD45RBhigh T cells were FACS sorted from CD160 knockout and wildtype littermate controls and transferred into Rag1-/- recipient mice for the induction of T cell transfer colitis. At peak of disease, CD4+ T cells were sorted from the colons for bulk RNA-seq.