In vitro effects of equine autologous conditioned serum and autologous equine serum on inflamed chondrocyte pellets

The aim of the study was to investigate the effects of autologous equine serum (AES) incubated for 24 h and autologous conditioned serum (ACS) on inflamed equine chondrocyte pellets in vitro. Inflammation was induced in vitro in 3-D pellet cultures of chondrocytes using recombinant equine IL-1β (5ng/ml). The pellet cultures were treated with culture media as unstimulated controls, IL-1β or IL-1β and one of the following treatments: AES, AES 24h and ACS. Chondrocyte pellets were harvested after 2 and 48 hours and total RNA was isolated. The effects were evaluated using GeneChip® Equine Gene 1.0 ST Array (Affymetrix Inc.).