Structural changes in microbial community of agricultural agrosoddy-podzolic soil under treatment of pesticides

Experiments were carried out in soil microcosms with treatment with three pesticides in preparative forms - imidacloprid, benomyl and metribuzin in single and tenfold application rates. For additional stimulation of microorganisms, a starch-mineral mixture was added to some variants. For all samples, high-throughput sequencing on the Illumina MiSeq platform of the V4 and ITS1 fragments of the 16S and 18S rRNA gene was carried out. As a result, it was possible to establish the characteristic changes in the structure of the fungal and bacterial communities, which are characteristic of pesticide treatment. The change in the relative representation of the genera Terrabacter, Kitasatospora, Streptomyces, Sphingomonas, Apiotrichum, Solicoccozyma, Gamsia, Humicola can be proposed as a marker of pesticide contamination. It is proposed to use these signs for mass assessment of the effect of pesticides on soil microbiota instead of classical integral methods, including within the framework of state registration of pesticides. It is also proposed to conduct research on the effect of pesticides on the soil microbiome during artificially initiated successions using the introduction of substrates.