Fibroblast-derived CSF1 is essential for intestinal macrophage maintenance

Tissue-resident macrophages are considered to be maintained through the interaction with the local microenvironment. However, precise mechansims which regulate the gut macrophage population remains elusive. We performed single cell RNA sequencing (scRNA-seq) of gut fibroblasts to characterize the cells which may potentially contribute to the local maintenance of macrophages in the gut. Overall design: PDPN-positive fibroblasts isolated from the small intestine of 4-week-old C57BL/6 mice (n=2) or from the colon of 9-week-old C57BL/6 mice (n=3) were subjected to scRNA-seq analysis, respectively.