Expression data from Butyroside D treated B16F10 cells [2uM]

Gene expression profiling reveals a potential role of Butyroside D in melanogenesis inhibition in B16F10 cells. B16F10 cells were murine melanoma cell line, treated with 2 μM Butyroside D for 48 h. Microarray gene expression profiling was conducted for two biological replicates RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis using Affymetrix’s GeneChip® System (Affymetrix Inc., Santa Clara, CA, USA) using Mouse Clariom S Array following the manufacturer’s instructions. Complementary RNA (cRNA) was synthesized using IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). The prepared samples were fragmented and labeled and prepared for hybridization using GeneChipTM Hybridization, Wash and Strain Kit. Each specific sample was injected into the GeneChip TM cartridge of Clariom S Array and incubated with rotation of 60 rpm for 16 h at 45℃ in the GeneChip® hybridization oven 645. Arrays were washed and stained with GeneChip® Fluidic station 450 and scanned with GeneChip® Scanner 3000.