The quantitative and phosphorylation proteomics analysis of mouse liver

Babesia microti is a tick-borne protozoan parasite that infects the red blood cells of mice, humans, and other mammals. Hosts infected with B. microti exhibit a series of clinical symptoms, including fever, anaemia, and haemoglobinuria, and multiple-organ injury. The infection can even result in death in patients with severe symptoms. Here, after BALB/c mice were infected with B. microti, liver tissues had serious injury and obvious swelling, cells were deformed, lipid droplets had formed in the cytoplasm, and organelles such as mitochondria also exhibited morphological changes. To further investigate the molecular mechanisms underlying liver injury and liver self-repair after B. microti infection, data-independent acquisition quantitative (DIA) proteomics was used to analyse changes in the expression and phosphorylation of proteins in liver tissues in the infection period and recovery period after BALB/c mice were infected with B. microti.