Effect of neonatal right ventricular pressure overload on gene expression and regions of open chromatin during postnatal heart development

To investigate the effect of pressure overload (PO) during postnatal right ventricular (RV) development, we established the RV PO model by conducting pulmonary artery banding (PAB) surgery on neonatal Sprague-Dawley (SD) rats. We then performed gene expression and chromatin openness profiling analysis using data obtained from RNA-seq and ATAC-seq of RV free walls from PO and sham-operated rats at postnatal day 21 (P21).为探究出生后右心室（right ventricular, RV）发育过程中的压力负荷（pressure overload, PO）效应，我们通过对新生Sprague-Dawley（SD）大鼠实施肺动脉环扎术（pulmonary artery banding, PAB），成功构建了右心室压力负荷模型。随后，我们采集出生后第21天（P21）的压力负荷组与假手术组大鼠的右心室游离壁样本，利用RNA测序（RNA-seq）与转座酶可及性测序（ATAC-seq）获取的相关数据，开展基因表达与染色质开放谱分析。