Effects of hexachlorocyclopentadiene (HCCPD). Daphnia magna

Sublethal effects of the flame retardant intermediate hexachlorocyclopentadiene (HCCPD) on the gene expression and protein activity of Daphnia magna Reference sample is a composite of equal amounts of total RNA (500 ng each) from n=3 culture media controls and n=3 methanol controls Overall design: Two-condition experiment: controls (methanol and cell culture) and three sublethal doses of HCCPD (0.138 ug/L, 1.38 ug/L, 13.8 ug/L). Biological replicates: 3 replicates each group.