4D Printed Fiber-Reinforced Scaffolds with High Elasticity and Sustained Estradiol Release for Uterine Tissue Regeneration

Fig.1     Schematical illustration for fabrication of electrospun fiber-reinforced PLATMC scaffolds.Fig.2     Characterization of PDA and PDA@E2 particles. Fig.3     Characterization of electrospun TPU and TPU-PDA fibers.Fig.4     The rheological properties of printing inks and characterization of printed scaffolds.Fig.5     The mechanical properties of fiber-reinforced scaffolds.Fig.6     In vitro degradation and E2 release of fiber-reinforced scaffolds.Fig.7    Biological properties of fiber-reinforced scaffolds.Fig.8    Shape morphing behavior of fiber-reinforced scaffolds.Fig.S1     The water contact angle of TPU, TPU-40PDA, and TPU-80PDA electrospun fibers.Fig.S2     The microscope images (bright field) showing the distribution of TPU-PDA fibers in 3D printed PLATMC filaments.Fig.S3     Dissipated energy in each hysteresis loop of PLATMC and PLATMC-30F scaffolds during cyclic tensile tests.Fig.S4     Application of Higuchi model for E2 release from (A) PLATMC-30F scaffolds and (B) PLATMC-TPU-PDA@E2 scaffolds.Fig.S5     SEM images showing BMSCs morphology attached on the PLATMC, PLATMC-TPU and PLATMC-30F scaffolds after cultured for 24h.